缺血再灌注对鼠脑血管内皮生长因子表达的影响Expression of Vascular Endothelial Growth Factor in Rat Brains Following Transient Global Ischemia
程敬君,匡培根
摘要(Abstract):
目的 了解血管内皮生长因子 (VEGF)在一过性全脑缺血再灌注鼠脑表达的动态变化。方法 采用Pulisnelli改良法四血管堵塞全脑缺血再灌注模型 ,用免疫组织化学方法观察了全缺血 15min再灌注 2h~ 14d时 ,VEGF表达的动态变化。结果 全脑缺血 15min再灌注 6hVEGF即可在大脑皮层、纹状体及丘脑等区域的血管内皮细胞表达 ,1d达高峰 ,一直持续到缺血后再灌注 3d。结论 脑缺血后再灌注可上调VEGF在大脑皮质、纹状体及丘脑等区域内皮细胞的表达 ,VEGF可能通过促进血管内皮细胞生长对缺血性神经元损伤起一定的作用。
关键词(KeyWords): 脑缺血再灌注;血管内皮生长因子;免疫组织化学
基金项目(Foundation): 军事医学科学院创新基金!资助项目 (No .2 0 12 0 4)
作者(Author): 程敬君,匡培根
Email:
DOI:
参考文献(References):
- [ 1]BrogiE ,SchahemanG ,WuTG ,etal.Hypoxin in ducedparacrineregulationbyvascularendothelialgrowthfactorreceptorexpression[J].JClinInvest ,1996 ,97:46 9 476 .
- [2 ]PulsinellWA ,BuchanAM .Thefour vesselocclusionratmodel:methodforcompleteocclusionofvertebralandcontrolofcollateralcirculation [J].Stroke ,1988,19:913 914.
- [3]FrraraN ,WinerJ,BurtonT .Aorticsmoothmusclecellsexpressandsecretevascularendothelialgrowthfac tor[J].GrowthFactors ,1991,5 :141 148.
- [4 ]BreierG ,AlbrechtU ,StererS ,etal.Expressionofvascularendothelialgrowthfactorduringembryonican giogenesisandendothelialcelldifferentiation[J].Devel opmentCamb ,1992 ,114:5 2 1 5 32 .
- [5 ]LadouxAa ,FrelinC .Hypoxizisastronginducerofvas cularendothelialgrowthfactormRNAexpressionintheheart[J].BiochemBiophysResCommun ,1993,195 :10 0 5 10 10 .
- [6 ]ConnollyDT ,HeuvelmanDM ,NelsonR ,etal.Tumorvascularpermeabilityfactorstimulatesendothelialcellgrowthandangiogenesis[J].JClinInvest ,1989,84:1470 1478.
- [7]MonacciWt,MerrillMJ ,OldfieldEH .Expressionofvascularpermeabilityfactor/vascularendothelialgrowthfactorinnormalrattissue[J].AmJPhysiol,1993,2 6 4:C995 10 0 2 .
- [8]BanaiS ,ShweikiD ,PinsonA .Upregulationofvascularendothelialgrowthfactorexpressioninducedbymyocar dialischemiaImplicationsforcoronaryangiogenesis[J].CardiovascRes,1994,2 8:1176 1179.