侯雪飞;龚仕涛;李锦超;李欣;

• 1:云南省干细胞和再生医学重点实验室昆明医科大学生物医学工程研究中心

摘要(Abstract)：

目的研究β-淀粉样前体蛋白/早老素1(amyloid precursor protein/presenilin 1,APP/PS1)双转基因阿尔茨海默病(Alzheimer disease,AD)模型小鼠不同年龄阶段自噬水平变化,及蛋白激酶B(protein kinase B,Akt)/哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)传统自噬信号通路的变化。方法选取4月龄、8月龄、12月龄APP/PS1转基因AD小鼠各4只,另选择同窝4月龄、8月龄、12月龄C57BL/6小鼠各4只为对照组。采用蛋白免疫印迹法检测各组小鼠海马自噬相关蛋白Akt、p-Akt、mTOR、p-mTOR、微管相关蛋白1轻链3(microtubule-associated protein 1 light chain 3,LC3)、选择性自噬接头蛋白(sequestosome-1,p62)的表达水平。结果与对照组比较,4月龄、8月龄APP/PS1转基因AD小鼠Akt、p-Akt、mTOR、p-mTOR、LC3、p62表达量均无统计学变化(均P>0.05),12月龄APP/PS1转基因AD小鼠p-Akt/Akt、p-Akt、LC3Ⅱ、LC3Ⅱ/LC3Ⅰ升高(P<0.05)。不同月龄APP/PS1小鼠海马LC3Ⅱ蛋白表达水平及LC3Ⅱ/LC3Ⅰ比较有统计学差异(P<0.05)。结论 APP/PS1转基因AD小鼠海马自噬与小鼠月龄有关。AD小鼠从12月龄开始出现自噬异常,Akt/mTOR信号通路异常激活。

关键词(KeyWords)： 阿尔茨海默病;自噬;淀粉样β蛋白前体;早老素1;Akt激酶信号通路;哺乳动物雷帕霉素靶蛋白

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金地区科学基金项目(31660273);; 云南省教育厅科学研究基金(2019Y0349);云南省教育厅科学研究基金指导性项目(2017zDX158)

作者(Author): 侯雪飞;龚仕涛;李锦超;李欣;

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DOI:

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