孙琳;王涛;韩慧琴;程艳;肖世富;

• 1:上海交通大学医学院附属精神卫生中心

摘要(Abstract)：

目的观察骨形态发生蛋白6(bone morphogenetic protein 6,BMP6)干预下β淀粉蛋白(amyloidβ,Aβ)毒性病变中大鼠海马神经元凋亡基因及凋亡关键酶的变化情况,探讨BMP6在Aβ致大鼠神经元损害中抗凋亡的机制。方法大鼠海马神经细胞原代培养,应用免疫荧光实验进行海马神经元鉴定。将细胞分为5组,Aβ组加入15μmol/L Aβ25-35孵育26h;Aβ+BMP6 100ng/mL组、Aβ+BMP6 150ng/mL组、Aβ+BMP6 200ng/mL组均加入Aβ25-35使终浓度为15μmol/L,孵育2h后分别加入100、150和200ng/mL BMP6,孵育24h;正常对照组(对照组)加入等量细胞培养液孵育26h。收集细胞后应用定量PCR法检测凋亡相关基因B细胞淋巴瘤基因2(B cell lymphoma 2,Bcl2)及Bcl2相关X蛋白(Bcl2associated X protein,Bax)含量,应用分光光度计法检测凋亡关键酶caspase 3、caspase 9活性。结果 Aβ+BMP6 100ng/mL组、Aβ+BMP6 150ng/mL组、Aβ+BMP6 200ng/mL组Bax mRNA含量(分别为0.7676±0.1312、0.5296±0.0185和0.2319±0.0756)均低于Aβ组(1.7081±0.2322)(P<0.05),caspase 3(1.8800±0.2031、1.7831±0.1839和1.2450±0.1529)、caspase 9(1.6804±0.0503、1.3310±0.0395和1.1500±0.0392)活性亦均低于Aβ组(caspase 3:2.4501±0.2056;caspase 9:1.9197±0.0858)(P<0.05)。各组间Bcl2mRNA比较含量差异无统计学意义(P>0.05)。结论 BMP6在Aβ致大鼠海马神经元损害中的抗凋亡作用可能与下调Bax基因表达和抑制caspase 3、caspase9活性有关。

关键词(KeyWords)： 阿尔茨海默病;β淀粉蛋白;骨形态发生蛋白;凋亡

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81301139);; 上海市自然科学基金(13ZR1435900)

作者(Author): 孙琳;王涛;韩慧琴;程艳;肖世富;

Email:

DOI:

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