糖皮质激素对活化后小胶质细胞株N9中MHC-Ⅱ和OX40L表达的影响Study of Glucocorticoid Effects on Expressions of MHC-Ⅱand OX40L in Activated Microglia N9 Cell Line
李茂全;王艳艳;余晓东;余争平;
摘要(Abstract)：
目的研究糖皮质激素对炎症刺激活化后的小胶质细胞株N9表面分子MHC-Ⅱ和OX40L表达的影响。方法脂多糖(LPS)联合小鼠γ-干扰素刺激N9细胞活化,采用RT-PCR、免疫荧光检测N9活化前后OX40L的表达。糖皮质激素及其受体阻断剂RU-486处理活化的N9细胞后,用流式细胞术(FACS)检测MHC-Ⅱ、OX40L的表达变化。结果N9活化前OX40L mRNA和蛋白均有组成性表达,活化后MHC-Ⅱ和OX40L表达显著上调(P<0.05),糖皮质激素处理后与活化组比较两分子表达下调(P<0.05),加入RU-486又可使两分子表达上调。结论小胶质细胞N9活化后MHC-Ⅱ、OX40L表达显著升高,而糖皮质激素可通过抑制小胶质细胞MHC-Ⅱ和OX40L表达拮抗小胶质细胞的抗原呈递功能,从而降低由T细胞活化介导的损伤效应。
关键词(KeyWords)： 糖皮质激素;小胶质细胞;MHC-Ⅱ;OX40L;多发性硬化
基金项目(Foundation): 国家自然科学基金(30400349);; 成都医学院院管基金资助项目
作者(Author): 李茂全;王艳艳;余晓东;余争平;
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参考文献(References)：
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