美解眠急、慢性致癫痫大鼠海马和大脑皮质糖皮质激素受体变化的研究Study of the Level of Glucocorticoid Receptor in Epileptogenic Rat Hippocampus and Cerebral Cortex
吴冰洁,顾平,王铭维,龚淑英
摘要(Abstract)：
目的 观察癫痫大鼠大脑皮质、海马糖皮质激素受体 (GR)的改变 ,阐明 GR在癫痫发生发展中的作用。方法 采用美解眠皮下注射方式建立大鼠癫痫模型。应用免疫组织化学 ABC法测定 60只 SD雄性大鼠大脑皮质及海马齿状回 GR阳性细胞数目的变化。结果 急性致癫痫组大鼠大脑皮质、海马齿状回 GR阳性细胞数显著低于对照组 (P <0 .0 0 1 ) ;慢性致癫痫组大鼠大脑皮质、海马齿状回 GR阳性细胞数显著高于对照组 (P <0 .0 1 )和急性致癫痫组 (P<0 .0 0 1 )。结论 大脑皮质和海马 GR水平的变化可能与癫痫发病有关。
关键词(KeyWords)： 癫痫;大鼠;大脑皮质;海马;糖皮质激素受体
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作者(Author): 吴冰洁,顾平,王铭维,龚淑英
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参考文献(References)：
- [1]JoelsM.Steroid hormones and excitability in themammalian brain[J].FrontNeuroendocrinol,1997,18(1):2-48.
- [2]DiehlGR,SmialowskiA,GotwoT.Developmentand persistence of kindlel seizures after repeatedinjection of pentylenetetrazol in rats and guinea pigs[J].Epilepsia,1984,259(4):506-509.
- [3]GouldE,WoolleyCS,McEwenBS,et al.Thehippocampal formation:morphological changesinduced by thyroid,gonadal and adrenal hormones[J].Psychoneuroendocrinology,1991,16(3):67-84.
- [4]MorrellMJ.Hormones and epilepsy through thelifetime[J].Epilepsia,1992,32(4):49-51.
- [5]KarstH,KloetER,JoelsM.Episodiccorticosteronetreatment accelerates kindling epileptogenesis andtriggers long-term changesin hippocampalCA1 cells,in the fully kindled state[J].EurJNeurosci,1999,11(3):889-898.
- [6]JoelesM,KloetER.Control of neuronal excitabilityby corticosteroid hormones[J].TINS,1992,15(1):25-27.
- [7]PrerettiS,GlannuarioAD,LoizzoA,et al.Dexamethasone prevents epileptiform activityinduced by morphinein vivoand vitro experiments[J].ExpTherap,1992,263:830-839.
- [8]王铭维,阮旭中,邹晓毅.大鼠致痫后血清和脑组织皮质醇水平测定及其意义[J].中国神经免疫学和神经病学杂志,1996,3(3):176-178.
- [9]王铭维,阮旭中,朱长庚.皮质醇对马桑内酯致痫大鼠大脑皮层、海马GABA免疫组化分布的影响[J].中华神经科杂志,1996,29(2):89-92.
- [10]NaotoM,JunA,FukukoK.Effects of intracerebroventricular administration of growth forhormone releasing factor on somatostatin secretioninto rat hypopysyiol portal blood[J ].Neuroendocrinology,1990,51:93-96.
- [11]EhlersCL,HenriksenSL,WangM,et al.Corticotrpin releasing factor produces increase in brainexcitability and convulsive seizures in rat[J].BrainRes,1983,278:332-336.
- [12]杨 钢.内分泌生理与病理生理学[M].天津:天津科学技术出版社,1996.749-758