重症肌无力患者外周血单个核细胞凋亡调控基因Bcl-2 mRNA的表达及意义The Expression and Significance of Apoptotic Modulating Gene Bcl-2 mRNA of Mononuclear Cells in the Peripheral Blood of Patients with Myasthenia Gravis
张士孟;张晨;刘敏;谢安木;丛志强;
摘要(Abstract):
目的探讨重症肌无力(MG)患者外周血单个核细胞(PBMC)凋亡调控基因Bcl-2mRNA表达水平与MG的关系。方法应用RT-PCR半定量方法检测45例MG患者(MG组)和30名健康对照组(NC组)PBMCBcl-2mRNA表达的相对含量,对MG患者病情按许贤豪绝对及相对评分法进行量化,激素治疗4周后测定Bcl-2mRNA表达水平。结果(1)MG患者PBMCBcl-2mRNA表达相对含量[(0.513±0.038)%]明显高于NC组[(0.371±0.045)%](P<0.05)。(2)病情以许贤豪绝对及相对标准评分为:轻、中、重及极重组,其PBMCBcl-2mRNA的相对含量分别为(0.466±0.047)%、(0.557±0.029)%、(0.561±0.052)%和(0.637±0.041)%,后3者明显高于轻度组患者(均P<0.05);Bcl-2mRNA的相对含量与其MG患者病情绝对评分呈明显正相关(r=0.767),且具有统计学意义(P<0.01)。(3)激素治疗后各组Bcl-2mRNA表达相对含量分别为(0.256±0.033)%、(0.475±0.045)%、(0.487±0.027)%和(0.481±0.051)%,与治疗前比较均明显降低(P<0.01);治疗后各组病情评分分别为4.29±1.70、20.12±10.79、27.29±11.50和32.20±16.80,均明显下降(P<0.01)。结论肾上腺皮质激素治疗后,MG患者随病情好转,伴PBMC的Bcl-2mRNA升高,提示:检测PBMC的Bcl-2mRNA有可能作为反映MG患者病情及免疫疗效的参数。
关键词(KeyWords): 重症肌无力;细胞凋亡;Bcl-2 mRNA
基金项目(Foundation): 青岛市科研基金资助项目(ZD01-078)
作者(Author): 张士孟;张晨;刘敏;谢安木;丛志强;
Email:
DOI:
参考文献(References):
- [1]Salakou S,Tsamandas AC,Bonikos DS,et al.The potential role of Bcl-2,Bax,and Ki67expression in thymus of patients with myasthenia gravis,and their correlation with clinicopathologic parameters[J].Eur J Cardiothorac Surg,2001,20(4):712-721.
- [2]Shiono H,Fujii Y,Okumura M,et al.Failure to down-regulate Bcl-2Proteinin thymic germinal center B cells in myasthenia gravis[J].Eur J I mmunol,1997,27(4):805-809.
- [3]王秀云,许贤豪,孙井宏,等.重症肌无力病人的临床绝对评分法和相对评分法[J].中华神经科杂志,1997,30(2):87-90.
- [4]李海民,窦科峰,李韧,等.肝癌组织中Fas和FasLm-RNA表达水平的变化及其意义[J].中国普外基础与临床杂志,2005,12(3):284-286.
- [5]Rinaudo MS,Suk,Falk IA,et al.Human interleukin-3receptor modutates bcl-2mRNA and protein levels through protein kinase C in TF-1cell[J].Blood,1995,86:80.
- [6]Thompson CB.Apoptosis in the pathogenesis and treat ment of disease[J].Science,1995,1267:1456.
- [7]Chang MK,Binder CJ,Miller YI,et al.Apoptotic cells with oxidation-specific epitopes are i mmunogenic and proinflammatory[J].J Exp Med.2004,200(11):1359-1370.
- [8]Strasser A,Harris AW,Cory S.Bcl-2trangene inhib-its T cell death and perturbs thymic self-censorship[J].Cell,1991,67(5):889-899.
- [9]Siegel RM,Katsumata M,Miyashita T,et al.Analysis of apoptosis in lymphoid organs and lupus disease in murine systemic lupus erythemato-sus[J].Clin Exp I mmunol,1996,105:306.
- [10]Onodera J,Nakamura S,Nagano I,et al.Upregulation of Bcl-2protein in the myasthenic thymus[J].Ann Neurol,1996,39-52.
- [11]Ohsako S,Hara M,Harigai M,et al.Expression and function of Fas antigene and Bcl-2in human systemic lupus eryhematosus lymphocytes[J].Clin I mmunol I mmuno-pathol,1994,73(1):109-114.
- [12]Graninger WB.Transcriptional overexpression of the protooncogene Bcl-2in patients with systemic lupus erythematosus[J].Wien Klin Wochenschr,1992,104(7):205-207.