JAK2-STAT3途径介导凝血酶诱导的小胶质细胞TNF-α的激活JAK2-STAT3 Signaling Pathway Mediates Thrombin-induced TNF-α Action of Microglia in vitro
黄承芳;黎钢;孙圣刚;
摘要(Abstract)：
目的探讨Janus激酶2/信号转导和转录激活子3(JAK2/STAT3)途径可否介导凝血酶诱导的小胶质细胞肿瘤坏死因子(TNF-α)激活。方法采用20U/mL凝血酶或AG490预处理(10μmol/L)+凝血酶(20U/mL)处理原代培养的小胶质细胞,经Western Blot法检测细胞Janus激酶1(JAK1)、磷酸化(P)-JAK1、P-JAK2、磷酸化-信号转导和转录激活子3(P-STAT3)、STAT3表达,酶联免疫吸附(ELISA)法检测血清上清液TNF-α水平。结果经凝血酶处理2~12h后JAK2、STAT3发生了磷酸化,3~48h后TNF-α表达增加。经JAK激酶抑制剂AG490预处理后再经凝血酶处理的小胶质细胞的JAK2和STAT3激活显著减少,且TNF-α释放被抑制。结论JAK2-STAT3途径可介导凝血酶诱导的小胶质细胞TNF-α激活。
关键词(KeyWords)： 凝血酶;小胶质细胞;Janus激酶2/信号转导和转录激活子3途径;肿瘤坏死因子
基金项目(Foundation): 国家自然科学基金资助项目(30500574)
作者(Author): 黄承芳;黎钢;孙圣刚;
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DOI:
参考文献(References)：
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