刘颖;刘华;武慧丽;辛晋敏;马太花;梁丽云;马存根;

• 1:山西大同大学脑科学研究所
• 2:山西大同大学脑科学研究所
• 3:山西大同大学脑科学研究所
• 4:山西大同大学脑科学研究所

摘要(Abstract)：

目的探讨实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)非敏感品系Wistar大鼠加用不同剂量牛型结核杆菌卡介苗(mycobacterium bovis bacillus calmette-guerin,M.bovis BCG)对EAE模型诱导的作用。方法将Wistar大鼠分为EAE1、EAE2、EAE3、EAE4共4组,然后分别用含4、8、10、12 mg M.bovis BCG/mL完全福(氏)佐剂(CFA)加豚鼠脑脊髓匀浆(GPSCH)制备不同浓度的完全抗原,分别免疫诱导Wistar大鼠EAE。观察不同剂量M.bovis BCG对诱导EAE模型发病情况、各项临床指标的影响,同时取脑和脊髓制成石蜡切片,行HE染色观察组织形态变化,行单核细胞趋化蛋白-1(MCP-1)mRNA原位杂交(ISH)计数其阳性细胞百分率。结果EAE3、EAE4组的发病率、体重减轻、临床症状评分、MCP-1 mRNA阳性细胞数显著高于EAE1、EAE2组(均P<0.05)。结论M.bovis BCG浓度的高低影响Wistar大鼠EAE的发病率。

关键词(KeyWords)： 实验性自身免疫性脑脊髓炎;牛型结核杆菌疫苗;单核细胞趋化蛋白-1

Abstract:

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基金项目(Foundation): 山西省2003年度回国留学人员科研基金资助项目(2003-69)

作者(Author): 刘颖;刘华;武慧丽;辛晋敏;马太花;梁丽云;马存根;

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参考文献(References)：

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