邵梅,陈生弟,刘振国

• 1:上海第二医科大学瑞金医院神经科
• 2:上海第二医科大学瑞金医院神经科
• 3:上海第二医科大学瑞金医院神经科 上海200025
• 4:上海200025

摘要(Abstract)：

目的　探讨β-淀粉样蛋白 ( Aβ)在形成淀粉样沉积前对体外培养的 PC1 2细胞的毒性作用。方法　应用流式细胞仪检测 Aβ的剂量依赖性毒性 ;应用 DNA琼脂糖凝胶电泳、DNA末端标记和电镜判断 Aβ损伤细胞的途径。结果　流式细胞仪检测发现随着 Aβ2 5 -35浓度的增加 ,PC1 2细胞的死亡率增加 ;加入 1 0 μmol/L Aβ2 5 -35 2 4 h后 ,经 TUNEL发现细胞核着色 ,并可见细胞核碎片 ;DNA琼脂糖凝胶电泳可见间隔 1 80～ 2 0 0 bp左右的梯度条带 ;电镜显示细胞核浓缩 ,胞浆内有空泡形成 ,胞浆内细胞器完好。结论　 Aβ在形成纤维状沉积前即对神经细胞有毒性作用 ,可促进神经细胞的凋亡。

关键词(KeyWords)： 可溶性淀粉样蛋白25-35片段;大鼠嗜铬细胞瘤细胞;凋亡;阿尔茨海默病

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基金项目(Foundation): 上海市科委自然科学基金面上资助项目 ( 99ZB14 0 19)

作者(Author): 邵梅,陈生弟,刘振国

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DOI:

参考文献(References)：

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