刘强;牛晓艳;汪超;王振海;

• 1:宁夏医科大学总医院神经内科

摘要(Abstract)：

目的观察人小胶质细胞在单纯疱疹病毒Ⅰ型(HSV-Ⅰ)感染后抗黏液病毒(Mx1)蛋白表达及干扰素-α(IFN-α)、IFN-β表达变化,初步探讨Mx1蛋白在单纯疱疹病毒(HSV)性脑炎中的可能抗病毒作用。方法应用流式细胞技术检测人小胶质细胞感染HSV-Ⅰ后4、12、48hMx1蛋白的相对表达,采用酶联免疫吸附测定(ELISA)方法检测细胞培养上清液中IFN-α、IFN-β的表达。结果 HSV-Ⅰ实验组各时间点人小胶质细胞Mx1蛋白表达、IFN-α及IFN-β表达均高于正常对照组(均P<0.01);HSV-Ⅰ实验组48h内人小胶质细胞Mx1蛋白表达及IFN-α、IFN-β表达随时间延长升高,且Mx1蛋白表达与IFN-α、IFN-β表达均存在正相关(r=0.632,P<0.01;r=0.714,P<0.01)。结论 HSV-Ⅰ感染可引起体外培养的人小胶质细胞Mx1蛋白表达上调及IFN-α、IFN-β分泌增加,Mx1蛋白可能参与了中枢神经系统抗HSV-Ⅰ过程。

关键词(KeyWords)： 抗黏液病毒蛋白;小神经胶质细胞;疱疹病毒1型,人;干扰素α;干扰素β

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金地区科学基金项目(81160151)

作者(Author): 刘强;牛晓艳;汪超;王振海;

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DOI:

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