刘剑锋;陈杉杉;冯伟;周寿红;

• 1:南华大学附属第二医院儿科
• 2:南华大学衡阳医学院生理学教研室
• 3:广西脑与认知神经科学重点实验室桂林医学院基础医学院生理学教研室

摘要(Abstract)：

目的观察硫化氢(H_2S)对脂多糖(LPS)诱导的小胶质细胞极化的影响并探讨JAK2/STAT3信号通路是否介导其作用。方法将培养的N9小胶质细胞随机分为对照组、LPS组、硫氢化钠(NaHS)组、LPS+NaHS组、蛋白酪氨酸激酶(JAK2)/信号转导和转录激活因子(STAT3)抑制剂α-氰基-(3,4-羟基)N-苄苯乙烯胺(AG-490)组、LPS+NaHS+AG-490组共6组,每组设3个复孔。采用MTT法检测各组细胞活力,采用ELISA法检测各组胶质细胞及培养上清液中白细胞介素1β(IL-1β)、IL-6、IL-4 IL-10水平,采用Western-blot检测各组细胞精氨酸酶1(Arg1)、诱导型一氧化氮合酶(iNOS)、磷酸化JAK2(p-JAK2)和磷酸化STAT3(p-STAT3)蛋白表达。结果与对照组比较,LPS组细胞Arg1蛋白及IL-4和IL-10表达水平增加,而iNOS蛋白、IL-1β、IL-6、p-JAK2蛋白和p-STAT3蛋白表达降低,p-JAK2/t-JAK和p-STAT3/t-STAT3比值降低(均P<0.05)。与LPS组比较,LPS+NaHS组细胞Arg1蛋白及IL-4和IL-10表达水平增加,而iNOS蛋白、IL-1β、IL-6、p-JAK2蛋白和p-STAT3蛋白表达降低,p-JAK2/t-JAK2和p-STAT3/t-STAT3比值降低(均P<0.05)。JAK2/STAT3信号通路抑制剂AG-490可减弱NaHS的作用。结论 H_2S可抑制LPS诱导的小胶质细胞M1型极化,其机制可能是通过JAK2/STAT3信号通路实现。

关键词(KeyWords)： 硫化氢;脂多糖;小神经胶质细胞;极化;JAK2/STAT3信号通路

Abstract:

Keywords:

基金项目(Foundation): 湖南省卫生健康委科研计划课题项目(C2019097);; 湖南省自然科学基金资助项目(No 2016JJ2110);; 广西脑与认知神经科学重点实验室开放课题(GKLBCN-20190105-02)

作者(Author): 刘剑锋;陈杉杉;冯伟;周寿红;

Email:

DOI:

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