大鼠脑缺血再灌组织中补体沉积及降纤酶干预的实验观察Study on Complement Deposition in Cerebral Ischemia-reperfusion Tissue and the Interference Effect of Defibrase
罗晓光,李绍英,曲恒春,刘亢丁,苏志强
摘要(Abstract):
目的本实验旨在观察脑缺血再灌损伤中是否有补体参与,其参与是否与组织坏死有关,蛇毒制剂降纤酶对其有无干预作用。方法制成大鼠大脑中动脉缺血2h再灌22h动物模型,运用免疫组化方法检测再灌组织中的补体沉积,并给予降纤酶处理,观察其干预效果。结果补体C3在缺血再灌组织中大量沉积,为强阳性;降纤酶干预后补体沉积减少,为弱阳性;正常组及假手术组脑组织中无补体沉积。经X2检验证实补体沉积与组织坏死关系密切(P<0.01)。给药组白细胞浸润较未给药组明显减少(P<0.05),组织损伤程度也相对较轻。结论脑缺血再灌组织中有大量补体参与,补体的参与同组织坏死有密切关系。蛇毒制剂降纤酶可减少缺血再灌组织中补体的沉积,从而削减白细胞浸润,减轻脑组织损伤程度。
关键词(KeyWords): 脑缺血;缺血再灌损伤;补体;蛇毒
基金项目(Foundation):
作者(Author): 罗晓光,李绍英,曲恒春,刘亢丁,苏志强
Email:
DOI:
参考文献(References):
- 1Longa EZ,Weinstein PR, Carlson S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats.Stroke, 1989,20: 84-91
- 2Mcmanus LM, William PK, Crawford MH, et al. Complement localization in ischemic baboon my-ocardium. Lab Invest, 1983, 48:436-447
- 3Pinckard RN,Orourbe RA,Crawford MH, et al. Complement localization and mediation of ischemic in-jury in baboon myocardium.J Clin Invest,1980,66:1050-1056
- 4Crawford MH,Grover FL,Kolb WP,et al.Complement and neutrophil activation in the pathogenesisof ischemic myocardial injury.Circulation,1988,78:1449-1458
- 5Fernandez HN, Henson PM, Otanl,A,et al.Chemotactic response to human C3a and C5a anaphyla-toxins evaluation of C3a and C5a leukotaxis in vitro and under simulated in vivo conditions.J Immunol,1978,120:109-115
- 6Kochanek PM,Hallenbeck JM.Polymorphonuclear leukocytes and monocytes/macrophages in thepathogenesis of cerebral ischemia and stroke.Stroke,1992,23:1367-1379
- 7Lachmann PJ,The control of homologous lysis.Immunol Today,1991,12:312-315
- 8Rossen RD,Michael LH,Kagiyama A,et al.Mechanism of complement activation after coronary arteryocclusion evidence that myocardial ischemia in dogs causes release of constituents of myocardial subcellu-lar origin that complex with human Clq in vivo.Circ Res,1988,62:572-584
- 9Hammerschmidt DE,Greenberg CS,Yamada O,et al. Cholesterol and atheroma lipids activate comple-ment and stimulate granulocytes:A possible mechanism for amplification of ischemic injury inatherosclerotic states.J Lab Clin Med, 1981, 98:68-77
- 10Vakeva A, Laurila P,Meri S.Regulation of complement membrane attack complex formation in my-ocardial infarction.Am J Pathol,1993,143:65-75
- 11Maroko PR,Carpenter CB,Chiariello M,et al.Reduction by cobra venom factor of myocardial necrosisafter coronary artery occlusion.J Clin Invest,1978,61:661-670