大鼠脑缺血再灌组织中补体沉积及降纤酶干预的实验观察Study on Complement Deposition in Cerebral Ischemia-reperfusion Tissue and the Interference Effect of Defibrase
罗晓光,李绍英,曲恒春,刘亢丁,苏志强
摘要(Abstract)：
目的本实验旨在观察脑缺血再灌损伤中是否有补体参与，其参与是否与组织坏死有关，蛇毒制剂降纤酶对其有无干预作用。方法制成大鼠大脑中动脉缺血2h再灌22h动物模型，运用免疫组化方法检测再灌组织中的补体沉积，并给予降纤酶处理，观察其干预效果。结果补体C3在缺血再灌组织中大量沉积，为强阳性；降纤酶干预后补体沉积减少，为弱阳性；正常组及假手术组脑组织中无补体沉积。经X2检验证实补体沉积与组织坏死关系密切（P＜0．01）。给药组白细胞浸润较未给药组明显减少（P＜0．05），组织损伤程度也相对较轻。结论脑缺血再灌组织中有大量补体参与，补体的参与同组织坏死有密切关系。蛇毒制剂降纤酶可减少缺血再灌组织中补体的沉积，从而削减白细胞浸润，减轻脑组织损伤程度。
关键词(KeyWords)： 脑缺血;缺血再灌损伤;补体;蛇毒
基金项目(Foundation):
作者(Author): 罗晓光,李绍英,曲恒春,刘亢丁,苏志强
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参考文献(References)：
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