张侃;王洪新;闫玉仙;唐江伟;高路燕;魏中平;

• 1:天津医科大学附属第四中心医院神经内科
• 2:武警后勤学院中心实验室

摘要(Abstract)：

目的探讨长春西汀对大鼠脑缺血再灌注损伤炎性反应机制的影响。方法将Wistar大鼠随机分为假手术组、脑缺血再灌注损伤组和长春西汀干预组,应用线栓法制备大脑中动脉闭塞2h后再灌注模型,分别于再灌注6h、24h、3d、7d采用2,3,5-氯化三苯基四氮唑(TTC)染色法检测脑梗死灶大小,采用干、湿重法检测脑组织含水量以评价脑水肿的程度,采用免疫组织化学和原位杂交的方法检测大鼠核转录因子(nuclear factor-kappa B,NF-κB)、肿瘤坏死因子α(TNF-α)的表达。结果与假手术组比较,模型组NF-κB的表达于再灌注6h即增高(P<0.05),TNF-α的表达于24h增高(P<0.05),3d时均达高峰(均P<0.05),7d时仍高于假手术组(均P<0.05);与模型组比较,长春西汀干预组于再灌注后24h、3d、7d时NF-κB和TNF-α表达降低。长春西汀干预组NF-κB各时点组内比较未见统计学差异(F=2.324,P>0.05)。与假手术组比较,模型组和长春西汀干预组脑组织水含量于脑缺血再灌注损伤6h即增高,3d时达到高峰(均P<0.05),7d时仍高于假手术组(均P<0.05)。结论长春西汀对大鼠脑缺血再灌注损伤炎性反应有明显的抑制作用。

关键词(KeyWords)： 长春西汀;再灌注损伤;炎症;NF-κB;肿瘤坏死因子-α

Abstract:

Keywords:

基金项目(Foundation): 天津市卫生局资助课题(2011ky04)

作者(Author): 张侃;王洪新;闫玉仙;唐江伟;高路燕;魏中平;

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DOI:

参考文献(References)：

• [1]Rahul D,Vivek S,Sidharth M,et al.Amelioration of intrace-rebroventricular streptozotocin induced cognitive dysfunctionand oxidative stress by vinpocetine-a PDE1inhibitor[J].EurJ Pharmacol,2009,620:49-56.
• [2]Schaller B,Graf R.Cerebral ischemia and reperfusion:the pathphysiologic concept as a basis for clinical therapy[J].J CerebBlood Flow Metab,2004,24:351-371.
• [3]Zotti T,Uva A,Ferravante A,et al.TRAF7protein promotesLys-29-linked polyubiquitination of kappaB kinase(IKKy)/NF-kappaB essential modulator(NEMO)and p65/relA pro-tein and represses NF-kappaB activation[J].J Biol Chem,2011,286(26):22924-22933.
• [4]Assed CA,Willing AE,Garbuzova-Davis S,et al.Lack of NF-κB P50exacerbates degeneration of hippocampal neurons afterchemical exposure and impairs leraning[J].Exp Neurol,2002,176(2):277-288.
• [5]Campbell KJ,Perkins ND.Regulation of NF-kappaB function[J].Biochem Soc Symp,2006,(73):165-180.
• [6]Xie C,Kang J,Ferguson ME,et al.Blueberries reduce pro-in-flammatory cytokine TNF-αand IL-6 production in mousemacrophages by inhibiting NF-κB activation and the MAPKpathway[J].Mol Nutr Food Res,2011,55(10):1587-1591.
• [7]Patyar S,Prakash A,Modi M,et al.Role of vinpocetine in ce-rebrovascular diseases[J].Pharmacological Reports,2011,63:618-628.
• [8]王春富,汤新强.长春西汀的临床应用进展[J].大连医科大学学报,2009,31(6):365-368.
• [9]Mokry J,Nosalova G.The influence of the PDE inhibitors oncough reflex in Guinea pigs[J].Bratisl Lek Listy,2011,112(3):131-135.
• [10]Alexandre E.Vinpocetine as a potent anti-inflammatory agent[J].PANS,2010,107(22):9921-9922.
• [11]Jeon KI,Xu X,Aizawa T,et al.Vinpocetine inhibites NF-κBdependent inflammation via an IKK-dependent but PDE-inde-pendent mechanism[J].Proc Natl Acad Sci USA,2010,107:9795-9800.
• [12]Ozer MK,Asci H,Oncu M,et al.Effects of pentoxifylline onamikacin-induced nephrotoxicity in rats[J].Renal Failure,2009,31(2):134-139.