单次地塞米松治疗对实验性脑出血后肺、脑组织的保护作用及机制The Protective Effects of Dexamethasone Single Administration on the Brainand Lung after Intracerebral Hemorrhage
刘庆;吴珊;
摘要(Abstract)：
目的研究单次地塞米松(Dex)治疗对脑出血(ICH)后肺、脑组织的保护作用及可能机制。方法采用立体定向技术自体血尾状核注入法制备SD大鼠ICH模型,将大鼠分为假手术组、ICH模型组和Dex治疗组。采用HE染色观察血肿周围组织与肺组织的炎症反应情况,采用透射电镜观察各组肺组织超微结构变化。采用RT-PCR法半定量检测NF-κB p65 mRNA及糖皮质激素受体α(GRα)mRNA在脑与肺中的表达情况。结果(1)ICH模型组脑水肿明显,血肿周围炎症反应重,肺组织损伤以间质性肺炎表现为主,肺泡结构破坏;Dex治疗组脑水肿较ICH模型组明显减轻,肺组织炎性反应减少。(2)NF-κB p65 mRNA表达,ICH模型组脑出血后血肿周围脑组织(0.44±0.08)和肺组织(0.69±0.16)均较假手术组(分别为0.27±0.05和0.26±0.04)增高(P<0.01),Dex治疗组脑(0.1 7±0.14)及肺(0.17±0.03)组织中NF-κB p65 mRNA均明显低于ICH模型组和假手术组(P<0.01);ICH模型组GRα mRNA表达水平,无论在脑组织(0.47±0.07)还是在肺组织(0.49±0.07)均低于假手术组(分别为0.64±0.03和0.65±0.13)(P<0.01),Dex治疗组脑组织(1.01±0.09)和肺组织(0.89±0.08)GRαmRNA表达较ICH模型组和假手术组均明显增强(P<0.01)。结论 ICH后早期、单次给予Dex治疗可减轻脑组织损害,亦可明显改善继发肺损害,其可能作用机制为Dex提高了GRα mRNA水平并抑制NF-κB p65 mRNA表达。
关键词(KeyWords)： 脑出血;核转录因子-κB;糖皮质激素;糖皮质激素受体α
基金项目(Foundation): 贵州省优秀科技教育人才省长专项基金资助项目(黔省专合字2006-58)
作者(Author): 刘庆;吴珊;
Email:
DOI:
参考文献(References)：
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