6-OHDA帕金森病大鼠模型清醒静止状态下基底节-皮层环路振荡性电活动的特征Characteristics of Resting-state Oscillatory Activity in the Basal Ganglia-cortical Loop of the 6-OHDA-lesioned Rat Models Simulating Parkinson Disease
李敏;朱俊玲;石美祥;方伟;高立;李星呈;李楠;王学廉;高国栋;
摘要(Abstract):
目的了解帕金森病(PD)模型大鼠在清醒静止状态下基底节-皮层环路的异常振荡性电活动。方法用6-羟基多巴胺(6-OHDA)脑内两点注射法建立PD大鼠模型,并经阿扑吗啡注射诱发旋转对模型进行评价。通过多导宏电极在体电生理记录技术结合视频录像,对可自由活动的正常大鼠和6-OHDA大鼠PD模型进行苍白球场电位和皮层脑电的多部位同时记录。功率谱分析和相干分析用于揭示各记录位点信号的频率成分以及不同记录位点神经元集群之间的功能连接和同步化。结果清醒静止状态下,苍白球场电位中存在特征明显的μ节律活动。与正常大鼠相比,PD模型大鼠苍白球场电位μ节律的持续时间明显延长;主运动皮层出现与苍白球同步的μ节律活动,特别是注射侧主运动皮层的μ节律被显著增强。结论μ节律是大鼠清醒静止状态下苍白球本身具有的一种生理性节律活动。6-OHDA对中脑多巴胺能神经元的损害可造成苍白球μ节律持续时间延长,还可改变基底节-皮层环路的功能连接,造成主运动皮层与苍白球之间异常同步的μ节律活动。至于此异常μ节律活动与PD静止性震颤的关系,尚需经进一步实验加以研究。
关键词(KeyWords): 帕金森病;大鼠;清醒静止状态;μ节律
基金项目(Foundation):
作者(Author): 李敏;朱俊玲;石美祥;方伟;高立;李星呈;李楠;王学廉;高国栋;
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